|
KMID : 0923620160160030176
|
|
Immune Network
2016 Volume.16 No. 3 p.176 ~ p.182
|
|
|
Roles of RUNX1 and PU.1 in CCR3 Transcription
|
|
Kong Su-Kang
Kim Byung-Soo
Hwang Sae-Mi
Chung Il-Yup
Lee Hyune-Hwan
|
|
|
Abstract
|
|
|
|
CCR3 is a chemokine receptor that mediates the accumulation of allergic inflammatory cells, including eosinophils and Th2 cells, at inflamed sites. The regulatory sequence of the CCR3 gene, contains two Runt-related transcription factor (RUNX) 1 sites and two PU.1 sites, in addition to a functional GATA site for transactivation of the CCR3 gene. In the present study, we examined the effects of the cis-acting elements of RUNX1 and PU.1 on transcription of the gene in EoL-1 eosinophilic cells and Jurkat T cells, both of which expressed functional surface CCR3 and these two transcription factors. Introduction of RUNX1 siRNA or PU.1 siRNA resulted in a modest decrease in CCR3 reporter activity in both cell types, compared with transfection of GATA-1 siRNA. Cotransfection of the two siRNAs led to inhibition in an additive manner. EMSA analysis showed that RUNX1, in particular, bound to its binding motifs. Mutagenesis analysis revealed that all point mutants lacking RUNX1- and PU.1-binding sites exhibited reduced reporter activities. These results suggest that RUNX1 and PU.1 participate in transcriptional regulation of the CCR3 gene.
|
|
|
KEYWORD
|
|
|
CCR3, RUNX1, PU.1, Transcription factor, Cis-acting element
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
   
|
|